Great work, congrats! I just have two questions. I believe you created the Thr122 mutant in vitro with a cell culture. How did you separate/purify the protein of your interest? Second, you mention that Thr122 works as a barrier. How is this inhibition tentatively working? by changing the folding of the protein or by selectively disturbing the lock-key interaction of the protein with the binding site? Thanks,
Abel Navarro
Great work, congrats! I just have two questions. I believe you created the Thr122 mutant in vitro with a cell culture. How did you separate/purify the protein of your interest? Second, you mention that Thr122 works as a barrier. How is this inhibition tentatively working? by changing the folding of the protein or by selectively disturbing the lock-key interaction of the protein with the binding site? Thanks,